abstract：:We have explored the adaptation of the cytochromes P450 (P450) of deep-sea bacteria to high hydrostatic pressures. Strict conservation of the protein fold and functional importance of protein-bound water make P450 a unique subject for the studies of high-pressure adaptation. Earlier, we expressed and purified a fatty-...

journal_title：Biotechnology and applied biochemistry

authors： Davydov DR,Sineva EV,Davydova NY,Bartlett DH,Halpert JR

更新日期：2013-01-01 00:00:00

abstract：:The structural underpinnings of O₂ activation in P450s have been limited by the inability to trap unstable oxy complexes in the crystalline state for structure determination. To date there are only two known oxy P450 structures. Even so, much is known about O₂ activation in P450cam and the role that redox partner bind...

journal_title：Biotechnology and applied biochemistry

authors： Poulos TL,Madrona Y

更新日期：2013-01-01 00:00:00

abstract：:Heparin is a widely used clinical anticoagulant that is prepared from pig intestine. A contamination of heparin in 2008 has led to a reexamination of animal-derived pharmaceuticals. A bioengineered heparin prepared by bacterial fermentation and chemical and enzymatic processing is currently under development. This stu...

journal_title：Biotechnology and applied biochemistry

authors： Suwan J,Torelli A,Onishi A,Dordick JS,Linhardt RJ

更新日期：2012-11-01 00:00:00

abstract：:The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphylococcal phage, was found to adsorb to a large number of Staphylococc...

journal_title：Biotechnology and applied biochemistry

authors： Biddappa AC,Sundarrajan S,Ramalinga AB,Sriram B,Padmanabhan S

更新日期：2012-11-01 00:00:00

abstract：:A novel iron superoxide dismutase (Fe-SOD) gene from Jatropha curcas was cloned and expressed in Escherichia coli BL21 (DE3). This recombinant enzyme was isolated by a combined procedure involving immobilized metal-ion affinity chromatography and ion-exchange chromatography. The apparent molecular mass of this purifie...

journal_title：Biotechnology and applied biochemistry

authors： Ou-Yang C,Cai F,Gao S,Niu B,Wang S,Chen F

更新日期：2012-09-01 00:00:00

abstract：:Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification w...

journal_title：Biotechnology and applied biochemistry

authors： Geng B,Wang M,Qi W,Su R,He Z

更新日期：2012-07-01 00:00:00

abstract：:Pancreatic duodenum homeobox protein-1 (PDX1) is a master regulatory gene in pancreatic development. Reprogramming of mesenchymal stem cells (MSCs) is a promising tool for producing insulin-producing cells. In this study, lentivirus harboring PDX1 (LV-PDX1) has been used for persistence gene expression in MSCs. The ob...

journal_title：Biotechnology and applied biochemistry

authors： Talebi S,Aleyasin A,Soleimani M,Massumi M

更新日期：2012-05-01 00:00:00

abstract：:The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress i...

journal_title：Biotechnology and applied biochemistry

authors： Abaci HE,Devendra R,Soman R,Drazer G,Gerecht S

更新日期：2012-03-01 00:00:00

abstract：:Most large-scale microbial production of recombinant proteins are based on Escherichia coli, yeasts, or filamentous fungi systems. Using eukaryotic hosts, antibody fragments are generally expressed by targeting to the secretory pathway. This enables not only efficient disulfide bond formation but also secretion of sol...

journal_title：Biotechnology and applied biochemistry

authors： Ezzine A,M'hirsi El Adab S,Bouhaouala-Zahar B,Hmila I,Baciou L,Marzouki MN

更新日期：2012-01-01 00:00:00

abstract：:We evaluated a new protocol for measurement of cyclosporine A (CsA) 2 H after dose (C2) on the V-Twin® analyzer. Imprecision, recovery, and linearity were determined using CsA-spiked blood pools. Accuracy was evaluated using specimens from renal, cardiac, and liver transplant patients, and results were compared with t...

journal_title：Biotechnology and applied biochemistry

authors： Morjana N,Yau H,Rea D,Ruttle D,Jones H,Siefring G Jr,Christenson R

更新日期：2011-11-01 00:00:00

abstract：:Tissue engineering has been conducted in the study of cardiovascular grafts for many years. Many obstacles have been overcome in this rapidly changing field, but one difficulty has remained until now: the large number of endothelial cells (ECs) needed for seeding the inner layer of bypass graft. Recent advances in end...

journal_title：Biotechnology and applied biochemistry

authors： Punshon G,Vara DS,Sales KM,Seifalian AM

更新日期：2011-09-01 00:00:00

abstract：:A sensitive label-free electrochemical aptasensor was successfully fabricated for thrombin detection with platinum nanoparticles (Pt) and blocking reagent horseradish peroxidase (HRP) as enhancers. A Nafion®-graphene-coated electrode was first modified with an electrochemical probe of methylene blue (MB) through elect...

journal_title：Biotechnology and applied biochemistry

authors： Sun T,Wang L,Li N,Gan X

更新日期：2011-07-01 00:00:00

abstract：:Immobilized metal affinity chromatography (IMAC) is widely used for the production of recombinant proteins for a variety of applications; however, a number of challenges are typically encountered by researchers depending on the properties of the specific proteins in question. Here, we describe technical issues we have...

journal_title：Biotechnology and applied biochemistry

authors： Belak ZR,Nair M,Ovsenek N

更新日期：2011-05-01 00:00:00

abstract：:The number of approaches to recombinant protein production in plants is greater than ever before. Development of these new and improved technologies as production platforms for plant-made pharmaceuticals has and will continue to create new commercial opportunities in the pharmaceutical sector. However, it is inevitabl...

journal_title：Biotechnology and applied biochemistry

authors： Paul M,Ma JK

更新日期：2011-01-01 00:00:00

abstract：:The present study elaborates a cost-effective and transfectant-free method for generating recombinant Bombyx mori (silkworm) nucleopolyhedrovirus in silkworm larvae and pupae by injecting invasive Escherichia coli carrying BmBacmid [BmNPV (B. mori nucleopolyhedrovirus)-Bacmid] into larval haemocoel. Up to 109 PFU (pla...

journal_title：Biotechnology and applied biochemistry

authors： Sun J,Yao L,Yao N,Xu H,Jin P,Kan Y

更新日期：2010-12-01 00:00:00

abstract：:The α-amylase from soya bean seeds was purified by affinity precipitation, resulting in approx. 20-fold purification with approx. 84% recovery. The purified α-amylase had an optimum pH of 5.5, optimum temperature of 75 °C, Arrhenius energy of activation of 6.03 kcal/mol (1 kcal≈4.184 kJ) and a Km of 2.427 mg/ml (starc...

journal_title：Biotechnology and applied biochemistry

authors： Prakash O,Jaiswal N

更新日期：2010-12-01 00:00:00

abstract：:The structural gene of elastase strain K (elastase from Pseudomonas aeruginosa strain K), namely HindIII1500PstI, was successfully sequenced to contain 1497 bp. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature elastase consists of 301 amino acids, with a molecular mass of 33.1 kD...

journal_title：Biotechnology and applied biochemistry

authors： Wong CF,Salleh AB,Basri M,Abd Rahman RN

更新日期：2010-09-01 00:00:00

abstract：:High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not ...

journal_title：Biotechnology and applied biochemistry

authors： Eriksson C,Schwenk JM,Sjöberg A,Hober S

更新日期：2010-06-09 00:00:00

abstract：:The use of the recombinant BoNT/A-Hc (carboxylic domain of the Clostridium botulinum neurotoxin heavy chain) has been proposed as a vaccine candidate for botulism. This fragment contains the principle protective antigenic determinant. In the present study, in order to maximize recombinant protein expression, after ver...

journal_title：Biotechnology and applied biochemistry

authors： Yari K,Fatemi SS,Tavallaei M

更新日期：2010-05-24 00:00:00

abstract：:Human thymosin alpha1 is an effective immune system enhancer for the treatment of cancer and viral diseases. Therefore the development of new methods for its synthesis is an urgent problem. In the present work, we propose an efficient scalable scheme for the production of recombinant thymosin alpha1. We used an expres...

journal_title：Biotechnology and applied biochemistry

authors： Esipov RS,Stepanenko VN,Beyrakhova KA,Muravjeva TI,Miroshnikov AI

更新日期：2010-05-19 00:00:00

abstract：:2,7'-Cyclolignans constitute a group of compounds regularly employed in clinical contexts due to their antiviral and anticancer properties as expressed in a number of already available designed drugs. Possibilities for other therapeutic developments are indicated. All commercial preparations actually originate from a ...

journal_title：Biotechnology and applied biochemistry

authors： Lautié E,Fliniaux MA,Villarreal ML

更新日期：2010-03-12 00:00:00

abstract：:The introduction of different methodologies for the construction and screening of complex protein libraries has provided powerful means in protein engineering for the development of molecules with desired traits. A challenge faced in many situations is to adapt a given methodology for efficient and rapid identificatio...

journal_title：Biotechnology and applied biochemistry

authors： Löfdahl PA,Nygren PA

更新日期：2010-03-05 00:00:00

abstract：:Recently, hPNAS-4 [human PNAS-4; also known as C1orf121 (Genbank accession number NP-057160)] was reported to be a novel pro-apoptotic protein in mammalian cells. However, at present there is little information about hPNAS-4 and its molecular mechanisms. In our present studies, the hPNAS-4 gene was first cloned into t...

journal_title：Biotechnology and applied biochemistry

authors： Deng H,Jiang Q,Liang S,Yan F,Hou S,Qian Z,Li J,Wen Y,Yang J,Wei Y

更新日期：2010-02-11 00:00:00

abstract：:The efficient enzyme-catalysed reaction of morpholines as model structures for bioactive compounds with para-dihydroxylated aromatic systems was carried out using the oxidoreductase laccase and atmospheric oxygen to produce eight novel morpholine-substituted aromatics. The laccase of Myceliophthora thermophila was use...

journal_title：Biotechnology and applied biochemistry

authors： Hahn V,Mikolasch A,Wende K,Bartrow H,Lindequist U,Schauer F

更新日期：2009-11-13 00:00:00

abstract：:The fission yeast Schizosaccharomyces pombe is a particularly useful model for studying the function and regulation of genes from higher eukaryotes. The genome of Sc. pombe has been sequenced, and DNA microarray, proteome and transcriptome analyses have been carried out. Among the well-characterized yeast species, Sc....

journal_title：Biotechnology and applied biochemistry

authors： Takegawa K,Tohda H,Sasaki M,Idiris A,Ohashi T,Mukaiyama H,Giga-Hama Y,Kumagai H

更新日期：2009-06-22 00:00:00

abstract：:Biologically active membrane proteins are difficult to isolate. Very often the isolated membrane proteins have low binding affinity or no biological integrity at all. Despite some success in isolation, one has to overcome the hurdles of obtaining sufficient quantity of the proteins and maintaining biological activity ...

journal_title：Biotechnology and applied biochemistry

authors： Wong RB,Lee AH,Rangan VS,Cheng KC

更新日期：2009-05-01 00:00:00

abstract：:The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',...

journal_title：Biotechnology and applied biochemistry

authors： Zhou ML,Shao JR,Tang YX

更新日期：2009-04-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of garlic (Allium sativum L.) on the diabetic nephropathy and oxidative stress induced by STZ (streptozotocin) in rats. Diabetes was induced in Male Sprague-Dawley rats by administering a single intraperitoneal injection of STZ (60 mg/kg of body weight). ...

journal_title：Biotechnology and applied biochemistry

authors： Mariee AD,Abd-Allah GM,El-Yamany MF

更新日期：2009-03-01 00:00:00

abstract：:Amyloglucosidase from Rhizopus mould and beta-glucosidase from sweet almond were employed for the preparation of phenolic and vitamin glycosides of vanillin, N-vanillylnonanamide, DL-dopa, dopamine, curcumin, alpha-tocopherol (vitamin E), pyridoxine (vitamin B(6)), ergocalciferol (vitamin D(2)), thiamin (vitamin B(1))...

journal_title：Biotechnology and applied biochemistry

authors： Charles RE,Ponrasu T,Sivakumar R,Divakar S

更新日期：2009-03-01 00:00:00

abstract：:Various approaches for removal of high-abundance components in body fluids are currently available. While most methods are constructed for plasma depletion, there is a need for body-fluid-specific strategies. The aim of the present study was to design an affinity matrix suitable for the depletion of high-abundance pro...

journal_title：Biotechnology and applied biochemistry

authors： Ramström M,Zuberovic A,Grönwall C,Hanrieder J,Bergquist J,Hober S

更新日期：2009-02-01 00:00:00

abstract：:c-Jun is a highly oncogenic transcription factor involved in the development of different types of cancer. In the present study we have generated c-Jun-binding-affinity proteins from a phage-displayed library of so-called 'Affibody ligands', developed by combinatorial engineering of a non-immunoglobulin-based scaffold...

journal_title：Biotechnology and applied biochemistry

authors： Lundberg E,Brismar H,Gräslund T

更新日期：2009-01-01 00:00:00

abstract：:Human lung carcinoma is a highly metastatic tumour that leads to a high death rate and in which lymphatic metastasis is an important early event. HYA (hyaluronan) is involved in the invasion of a variety of tumour cells. Layilin, a specific and novel HYA receptor, was detected in the human lung cell line A549 in our p...

journal_title：Biotechnology and applied biochemistry

authors： Chen Z,Zhuo W,Wang Y,Ao X,An J

更新日期：2008-06-01 00:00:00

abstract：:A simplified and low-cost method, boiling lysis, was developed and used to purify the truncated DNA polymerase Stoffel fragment after overproduction of the enzyme in Escherichia coli. The method is based on the thermostable properties of the Stoffel fragment. Conditions such as the boiling time and number of cycles em...

journal_title：Biotechnology and applied biochemistry

authors： Yang Z,Ding Y,Zhang Y,Liu F

更新日期：2008-06-01 00:00:00

abstract：:The combination of IL-2 (interleukin-2) and GM-CSF (granulocyte/macrophage colony-stimulating factor) has been broadly studied in antitumour immune therapy, but its efficacy is uncertain. To better exert the activities of the two cytokines and study them in a mouse model, we have constructed a bifunctional protein, hI...

journal_title：Biotechnology and applied biochemistry

authors： Wen Q,Ma L,Luo W,Zhou MQ,He D,Lin Y,Wu ZQ,He XW,Wang JF,Wang XN

更新日期：2008-05-01 00:00:00

abstract：:In the present study, the growth and key metabolic features of a gene-transfected Drosophila melanogaster (fruitfly) S2 (Schneider 2) cell population (S2AcRVGP cells), cultured in Sf900-II medium, have been evaluated to provide substantial support for the development of a bioprocess to produce RVGP (rabies-virus glyco...

journal_title：Biotechnology and applied biochemistry

authors： Swiech K,da Silva CS,Arantes MK,dos Santos AS,Astray RM,Pereira CA,Suazo CA

更新日期：2008-01-01 00:00:00

abstract：:Agrobacterium rhizogenes-mediated hairy-root cultures are a very promising alternative to the biotechnological exploitation of plant cell cultures. Their characteristic capacity for secondary-metabolite production, inherent genetic stability reflected in stable productivity and the possibility of genetic manipulation ...

journal_title：Biotechnology and applied biochemistry

authors： Mishra BN,Ranjan R

更新日期：2008-01-01 00:00:00

abstract：:Talpha1 (thymosin alpha 1) is important in treating immunodeficiency and other diseases. In order to study the feasibility of expressing Talpha1 in plants, as the first attempt, we designed and synthesized the Talpha1 gene according to the plant codon usage preference and constructed the 4xTalpha1 concatemer (four cop...

journal_title：Biotechnology and applied biochemistry

authors： Chen Y,Zhao L,Shen G,Cui L,Ren W,Zhang H,Qian H,Tang K

更新日期：2008-01-01 00:00:00

abstract：:A three-layered simulation methodology is described that rapidly evaluates biomanufacturing process options. In each layer, inferior options are screened out, while more promising candidates are evaluated further in the subsequent, more refined layer, which uses more rigorous models that require more data from time-co...

journal_title：Biotechnology and applied biochemistry

authors： Chhatre S,Francis R,O'Donovan K,Titchener-Hooker NJ,Newcombe AR,Keshavarz-Moore E

更新日期：2007-10-01 00:00:00

abstract：:The aim of the present study was to determine both the minimal and the optimal conditions under which heat treatment is effective for enhancing 3D (three-dimensional)-like cell proliferation. C3H10T1/2 mouse fibroblasts were cultured with hydroxyapatite granules for 10 weeks after heat treatment at 40, 41.5, 43, 44 or...

journal_title：Biotechnology and applied biochemistry

authors： Hiragami F,Akiyama J,Koike Y,Kano Y

更新日期：2007-05-01 00:00:00

abstract：:Production of His-BbST [hexahistidine-tagged BbST (bubaline somatotropin)] by auto-induction in high-density shake-flask cultures coupled with a single-step, on-column purification and refolding strategy is described here. To optimize expression of BbST, different media and expression conditions were tested. The highe...

journal_title：Biotechnology and applied biochemistry

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2007-05-01 00:00:00